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| Registros recuperados : 1 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
18/11/2020 |
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Data da última atualização: |
18/11/2020 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SANTOS, F. C.; ROSA, S. D. V. F. da; GROTEWOLD, E.; PADILHA, L. |
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Afiliação: |
The Ohio State University; STTELA DELLYZETE VEIGA F DA ROSA, CNPCa; The Ohio State University; LILIAN PADILHA, CNPCa. |
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Título: |
Efficiency of coffee seeds rna extraction protoco using rna integrity number analysis. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 25., 2014, Armenia. Leveraging knowledge for coffee sustainability: proceedings. Armenia: Association for Science and Information on Coffee, 2014. p. 273-274. |
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Idioma: |
Português |
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Conteúdo: |
Coffee is one of the most important agricultural products in the world market. The mode of coffee processing, whether wet or dry, determines the flavor characteristic and also the seed quality. Quality RNA isolation is a mandatory requirement for studies of gene expression, including reverse transcriptase (RT), real-time quantitative PCR (RT-qPCR), construction of cDNA libraries, or microarray analyses. Due to the presence of secondary metabolites, polysaccharides, and polyphenols, standardization of a quality RNA extraction for different coffee plant tissue is very difficult. Also, getting high-quality RNA may be complicated because of RNA susceptibility to RNase degradation. |
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Thesagro: |
Café; Extração; RNA; Semente. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/217961/1/PB240.pdf
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Marc: |
LEADER 01397nam a2200193 a 4500
001 2126783
005 2020-11-18
008 2014 bl uuuu u00u1 u #d
100 1 $aSANTOS, F. C.
245 $aEfficiency of coffee seeds rna extraction protoco using rna integrity number analysis.$h[electronic resource]
260 $aIn: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 25., 2014, Armenia. Leveraging knowledge for coffee sustainability: proceedings. Armenia: Association for Science and Information on Coffee, 2014. p. 273-274.$c2014
520 $aCoffee is one of the most important agricultural products in the world market. The mode of coffee processing, whether wet or dry, determines the flavor characteristic and also the seed quality. Quality RNA isolation is a mandatory requirement for studies of gene expression, including reverse transcriptase (RT), real-time quantitative PCR (RT-qPCR), construction of cDNA libraries, or microarray analyses. Due to the presence of secondary metabolites, polysaccharides, and polyphenols, standardization of a quality RNA extraction for different coffee plant tissue is very difficult. Also, getting high-quality RNA may be complicated because of RNA susceptibility to RNase degradation.
650 $aCafé
650 $aExtração
650 $aRNA
650 $aSemente
700 1 $aROSA, S. D. V. F. da
700 1 $aGROTEWOLD, E.
700 1 $aPADILHA, L.
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Registro original: |
Embrapa Café (CNPCa) |
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